Yeah! The varied pitch moving up+ down at the end of the note is tremolo^^, I'm glad what I said made sense to you! haha, sometimes it's hard to describe what you're hearing... it's like trying to explain the color blue to a blind person lol

I am pretty sure that tremolo effects can be added via software (I think vocaloid can do this? I'm not sure on things like Kontakt etc though), but the note still sounds 'synthetic'; there's no real variance between the speed of the tremolo etc.

Music software is coming a long way, I'll be impressed when they finally master making synthesized sounds indistinguishable (haha I love that word..) from natural sounds, ie. a synth vs a violin or flute